Antigen Specific Antibody Secretion Protocol (V1.2)
Last Modified: 5/10/2023
This protocol covers a single-cell screening assay to sort out antigen-specific IgG secreting cells from a mixed population of antibody secreting cells. The assay is performed on mouse hybridoma cells or plasmablasts / plasma cells isolated from mice spleens or bone marrow with Nanovials sized for leukocytes (Product #NV1-35-BT-01).