Single-Cell Cytokine Profiling General Protocol (V2.0)
Version 2.0
Last Modified: 08/08/2023
This protocol provides guidance on the general workflow for single-cell cytokine secretion profiling using Nanovials (Product#:NV1-35-BT-01 or NV1-35-BG-01). This protocol was specifically developed on human primary T-cells isolated from PBMCs and IFNγ secretion, some optimization may be required for specific cell types and cytokines.